Prolactin Immunoreactivity in Rheumatoid Factor-Containing Specimens: Is it Prolactin, Macroprolactin or Assay Interference?
نویسنده
چکیده
Specimens containing Rheumatoid factor (RF) were examined for possible interference in Prolactin (PRL) measurement. PRL levels following polyethylene glycol (PEG) treatment were significantly lower than in untreated RFpositive samples (p<0.001; n=43) and spurious hyperprolactinemia was indicated in 7 percent. Gel filtration chromatography suggested that the PRL-like immunoreactivity was from assay interference rather than a macroPRL complex and that PEG treatment successfully removed the interference. It is important that laboratories consider explanations other than macroPRL when interpreting PRL results following PEG treatment.
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Acs:180 Results Obtained for Macroprolactinemic Samples Multiplexed Mutagenically Separated Pcr: Simulta- Neous Single-tube Detection of the Factor V R506q (g1691a), the Prothrombin G20210a, and the Methyl- Enetetrahydrofolate Reductase A223v (c677t) Variants
(range, 250% to 86%) indicates a highly variable, sampledependent response of the ACS:180 assay to macroprolactin. The great disparity of values observed when comparing results from macroprolactinemic samples measured by the Elecsys or the Immulite assay with the results obtained by a low-reading method such as ACS:180 may reflect variation in the structure of macroprolactin. Macroprolactin is ...
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